FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264798
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC95323.5997250734522166No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT86733.2753268529218498No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT76282.8806864100182024No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC72542.73944667255795No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT71152.686953828956412No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT63822.4101390493885906No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT59792.2579475675798157No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA55952.112931366551107No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA55882.1102878420531876No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC55292.0880067069992974No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC52671.9890633615057516No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG50951.924108188128309No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC50631.91202350470925No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA50191.8954070650080437No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA49801.8806788570910657No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA48691.8387601114812044No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA48041.814213098286241No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA44721.6888345078135032No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC44401.6767498243944439No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC44241.6707074826849146No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA44151.6673086654733043No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC43101.6276557980045168No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39161.478863133407352No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA37001.3972915203287033No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA36461.3768986170590412No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32691.2345259405282516No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA32681.2341482941714061No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC28251.0668509580888073No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC27991.057032152810822No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT25790.9739499543047907No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC24530.9263665133422458No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC24070.9089947809273484No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC20080.7583138845459558No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC19330.7299904077825361No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17880.6752316860399248No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC17120.6465305629196595No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC16580.6261376596499973No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA15770.5955483047455041No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT15670.5917718411770482No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA15450.5834636213264451No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15060.5687354134094668No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT15000.5664695352683933No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC14350.5419225220734295No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAAACAAATAAATAACT13560.5120884598826275No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13360.5045355327457156No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC12650.47772264140967835No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA12380.4675261897748473No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA10630.40143807732686804No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA9700.36631696614022763No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9170.3463017092274111No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8390.31684529339345463No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA7740.2922982801984909No Hit
AAAAATCCTAAAAACCCCAAAACACAAATTCCAAAATCTACCTAATGTAG7250.27379360871305675No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6970.26321951072138006No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6830.25793246172554174No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA6580.24849130280440185No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6210.2345183876011148No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC5360.2024184472692392No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4910.1854243612111874No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT4660.17598320229004752No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC4630.17485026321951072No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4550.17182909236474597No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA4490.16956321422367238No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4210.15898911623199571No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3950.14917031095401023No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3600.13595268846441438No Hit
AATACCTTAACTCCCTAAAACCCAAGTATAAATCAAAAATATCCCAAAAC3540.13368681032334082No Hit
AAACTACAAAATCAAAACCAACCCAACTAAATAAAAACTTACCCAACCCC3420.12915505404119365No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC3420.12915505404119365No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAACTCACCCA3330.1257562368295833No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT3220.12160212690428177No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3080.1163150779084434No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG2970.11216096798314186No Hit
CCTAAAAAAACAACACTCCAAACACCACTCCAAACCCCACTCTATCTTAC2950.11140567526945068No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC2850.10762921170099472No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC2700.10196451634831079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)