FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279928
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT83652.98826841187734No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC74912.6760452687834015No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT69572.4852819296390503No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC68752.4559886828041497No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT68202.4363407733417164No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT65102.325598010917093No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC63132.2552227715698323No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA60642.166271326912635No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA57002.036237889743077No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA55921.9976565402532078No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA55611.9865822640107456No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC55271.974436283615787No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT54251.9379983424309108No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA53281.9033465748335285No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC48191.7215141036266468No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA48101.718298991169158No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT46741.669715069589323No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC45251.616487096682004No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA43191.5428967448772541No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC43131.5407533365722614No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA42861.5311079991997942No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC39781.4210797062101683No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39211.4007173273127376No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA37991.3571346917778857No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC36711.3114086479380413No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA34991.2499642765282502No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32661.1667285873510331No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT31081.110285501986225No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29721.0617015804063903No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA28391.0141893629790517No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC28021.0009716784315967No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA27030.9656054413992169No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT26270.9384556028693092No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC25510.9113057643394016No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG22070.7884170215198194No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC19750.7055385670601011No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA18910.6755308507902031No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT16820.6008687948329571No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC16080.574433425738047No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15800.5644308536480809No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC14700.5251350347232145No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC13970.4990569003458032No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13880.4958417878883141No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA13850.49477008373581777No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA12300.4393987025235061No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAGCCACGCTACCTT11090.39617330170615306No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT10020.35794918693378297No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC9140.32651253179388984No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA9090.32472635820639595No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA8880.3172244291389214No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT7950.2840016004115344No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC7800.2786430796490526No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT7500.26792603812408905No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC7330.2618530479266097No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC6960.24863536337915462No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC6810.24327684261667287No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCGCCAACAT6570.234703209396702No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6500.23220256637421052No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAAACGACACA6400.22863021919922263No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA6220.22219999428424453No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC5410.1932639821668429No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5230.18683375725186477No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5000.17861735874939272No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4740.16932925609442429No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4680.16718584778943157No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACACTAAAACCTTCATC4390.15682604098196679No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4280.15289645908948016No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC4250.1518247549369838No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4110.1468234688920008No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4040.1443228258695093No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA3710.1325340801920494No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCGAAACCCAAACGCCTCCAT3650.13039067188705666No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA3640.13003343716955787No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3500.1250321511245749No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA3490.1246749164070761No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3470.12396044697207853No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3290.1175302220571004No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA3130.11181446657711983No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3110.11109999714212226No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAACGACACAA2880.10288359863965019No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC2820.10074019033465749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)