FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287860
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC124824.336135621482665No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT93313.2415062877787815No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC80152.7843396095324118No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT79462.7603696241228373No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA79132.748905718057389No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA77302.6853331480580835No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC76802.667963593413465No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG74932.60300145904259No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC72672.5244910720489124No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA68012.3626068227610646No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA62752.1798791078996733No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT60632.106232196206489No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC59832.0784409087750992No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT57441.9954144375738205No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT56531.963801848120614No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC56311.956159244076982No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA53281.8508997429305913No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT52611.827624539706802No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA50021.7376502466476758No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC48931.6997846175224067No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT46091.601125547140971No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC42601.4798860557215312No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC41821.4527895504759258No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA41351.436462169109984No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA39861.3847008962690195No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT36601.2714513999861043No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC32911.1432640867088169No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC32181.117904536927673No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC30911.0737858681303412No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC30891.0730910859445564No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA27750.9640102827763496No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA26490.92023900507191No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19810.6881817550198013No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT18530.6437156951295769No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC18090.6284304870423123No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC17580.6107135413048009No Hit
CCAACTAAAACTCAACATAATATCAAACACAAACCCTTCCAAATTCTTTT13240.45994580698950877No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA13110.45542972278190785No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA11860.4120058361703606No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA11830.4109636628916834No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAGCACC11600.40297366775515875No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10920.379351073438477No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA9260.3216841520183423No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA9190.3192524143680956No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA9020.3133467657889252No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCAACC8510.29562982005141386No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA8440.29319808240116724No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA6420.22302508163690682No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG6210.2157298686861669No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACACCAATACAC6110.2122559577572431No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCATACTCCCTTCTAGCACC5650.19627596748419374No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5310.18446467032585284No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTACCTACTTCTACCTCCGCCT5290.1837698881400681No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5100.1771694573751129No Hit
AATAAAAAACTCCCTCTTCCAAAAATTATTTAAAATTCAAAACCTTATTT4550.1580629472660321No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACACTTCACCAAAAATACTAAA4360.1514625165010769No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4260.14798860557215313No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA4050.14069339262141317No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC3870.1344403529493504No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3690.12818731327728758No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3620.12575557562704093No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA3170.11012297644688389No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3130.1087334120753144No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT3010.10456471896060585No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC2950.10248037240325157No Hit
CACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA2920.10143819912457445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)