FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315997
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC85062.691797706940256No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT81192.5693281898245868No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT79792.5250239717465672No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC76742.4285040680765957No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT74992.373123795479071No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA65742.0803994974635835No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA64952.055399260119558No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA63542.010778583340981No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC61751.954132475941227No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT57341.8145741889954652No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA57071.8060298040804186No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC56481.7873587407475386No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA56361.7835612363408513No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC55331.7509659901834511No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC54091.7117251113143481No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT52711.6680538106374427No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC52571.6636233888296408No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA49481.5658376503574403No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA47961.517735927872733No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC46741.4791279664047443No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG45421.437355417931183No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA44931.421848941603876No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC44641.4126716392877148No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA42711.3515951100801589No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC42231.3364050924534094No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA38461.2171001623433133No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT37571.188935337993715No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT34191.0819722972053532No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT29190.9237429469267113No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA27840.8810210223514781No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT27460.8689955917303013No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA26560.8405143086801456No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC26110.8262736671550679No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA26100.8259572084545107No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC24970.7901973752915376No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATC23370.7395639832023722No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA22230.7034876913388418No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21140.668993692978098No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC19860.6284869793067656No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA18770.5939929809460217No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17880.5658281565964234No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA16670.5275366538289921No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACGCCAACAT16480.5215239385184036No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT14570.4610803267119624No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA13880.4392446763735099No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC13580.42975091535679133No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACACAAACAAACC13070.41361152162836984No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12230.38702899078155806No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11880.3759529362620531No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11810.37373772535815214No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11730.37120605575369386No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA9570.30285097643332054No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT9360.2962053437216176No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC9220.2917749219138156No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8540.27025573027592037No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8420.2664582258692329No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8050.2547492539486134No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA7400.23417943841238997No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT6970.2205717142884268No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC6780.21455899897783842No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6760.2139260815767238No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6710.2123437880739374No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC6660.21076149457115098No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCGCCAAACCCCCACAAAAC6340.2006348161533179No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCCAAAATAA6310.19968544005164604No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA6240.19747022914774506No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC5750.18196375282043817No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5620.17784978971319346No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4610.1458874609569078No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAACAACACA4510.14272287395133498No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4000.12658348022291352No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCGACAT3930.12436826931901251No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3810.12057076491232512No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAACAACACAA3590.11360867350006489No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3560.11265929739839302No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA3550.11234283869783575No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3510.11107700389560661No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3180.10063386677721622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)