FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274136
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC81962.989756908979485No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT77232.8172148130854757No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT75672.7603087518603906No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT69152.5224705985350337No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC63632.3211106895847315No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT63022.298858960515948No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC59912.185411620509528No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC58292.1263168646219395No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA56522.0617503720780928No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA56372.0562786354218345No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA56262.0522660285405783No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA53401.9479382496279218No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC50011.8242770011964864No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC46761.7057227069775587No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA44641.6283888289024426No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC44521.624011439577436No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC44311.6163510082586745No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA43311.5798727638836199No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT43241.577319286777366No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT42041.5335453935273005No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA39351.435418916158403No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA37901.382525461814574No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA37631.372676335833309No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC37581.3708524236145563No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT36121.3175941868269763No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA34081.2431785683018648No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33641.2271281407768408No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC32171.1735051215455103No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC31701.1563603466892345No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA31371.1443225260454664No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA31071.1333790527329501No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA28961.0564099571015846No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT28961.0564099571015846No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA27911.018107800507777No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA22360.8156535442262235No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT21700.7915779029386875No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21690.7912131204949368No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC18830.6868853415822803No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA17910.65332535675723No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT17080.6230484139259346No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA16490.6015262497446523No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC16250.5927714710946391No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG15480.5646832229258469No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA14710.5365949747570549No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14540.5303936732132957No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC9850.359310707094289No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC9590.34982636355677477No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC8450.3082411649692124No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7300.2662911839378994No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA6600.24075641287536112No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT6550.2389325006566084No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA6200.22616511512533927No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACGCCAAACCCCCACAAAAC6150.2243412029065865No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC6060.2210581609128316No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5500.20063034406280095No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5020.18312078676277468No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4910.17910817988151867No Hit
ATCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4880.17801383255026704No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4820.17582513788776374No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4800.17509557300026266No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4620.16852948901275278No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCGAAAAAAAAAACGACACA4370.1594099279189891No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4200.15320862637522983No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATGCC3800.13861732862520793No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAGTTCAAAACCTTATT3450.1258499430939388No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3420.12475559576268713No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC3380.12329646598768494No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATGAATAACT3350.1222021186564333No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3350.1222021186564333No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3290.12001342399393003No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC3180.11600081711267402No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3080.11235299267516852No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC2930.10688125601891031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)