FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299868
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC89572.9869809382795096No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT86172.873597716328518No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT85422.848586711486387No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT79082.637160350554244No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC72712.424733549428415No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT66992.2339829524990997No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT65752.1926314244934435No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA62782.093587845318607No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA61902.0642415996371737No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA60262.0095508690490482No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC58841.9621966998812812No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC55821.8614857203836357No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA54751.8258033534755291No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA52431.7484359784972054No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC52181.7400989768831618No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC51651.72242453346139No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA50431.6817399655848573No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT48291.6103752317686448No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA48131.6050395507356567No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA47321.578027665506156No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT45481.5166673336267957No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC40311.344258140248376No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39271.3095762135339548No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA39081.303240092307282No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC37501.2505502421065269No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA35961.199194312164019No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG35841.195192551389278No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC35451.1821868288713702No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA34861.1625115050622274No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT33561.119159096669201No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC31561.052463083756853No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA26350.8787199701201863No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA25290.8433710832766417No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC24710.8240292395320608No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23470.782677711526405No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT22300.7436605439726813No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC21820.7276535008737177No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC18280.6096015580188616No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15520.5175610601998213No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC14600.48688089426014114No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC14400.48021129296890636No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC13970.46587165019275145No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA12560.4188509610895461No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC12210.40717915882988515No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11080.36949591153440847No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA9710.32380914268945005No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9380.3128043005589126No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA9140.3048007790094308No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8910.2971307375245108No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA8880.2961302973308255No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA8720.2907946162978377No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACGAACAAACC6960.2321021249349714No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT6590.21976336254618697No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6490.21642856190056958No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6470.21576160177144613No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC6360.21209332106126694No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5860.19541931783317992No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACGCCAACAT5840.19475235770405644No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAACACC4930.16440567182893806No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT4830.16107087118332064No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC4410.14706470847172756No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4370.1457307882134806No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4250.14172902743873972No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA3990.13305854576013446No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3960.13205810556644926No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC3840.12805634479170835No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA3610.12038630330678832No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3490.11638454253204743No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACGC3330.1110488614990596No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3110.10371230007870129No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG3000.10004401936852216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)