FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262336
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT131245.002744571846792No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC93553.566037448158087No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT90673.4562545742864113No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA83023.1646438155647716No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA81743.1158514271773603No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA71902.740759941449134No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC68902.626402781166138No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT68512.6115363503293487No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT68482.6103927787265184No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC68142.5974323005611124No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC66422.5318675286655283No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA59612.2722767748231276No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC56532.1548700902659186No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA54402.0736765064649916No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC53972.057285313491095No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC49571.889561478409368No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA48741.857922664064406No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA48481.848011710173213No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT44031.6783819224201024No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC43351.6524609660892902No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT42611.6242528665528178No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT41861.595663576482069No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC41431.5792723835081728No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC37901.4447121249085144No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA37721.4378506952915344No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC34591.3185380580629422No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC26841.0231153939985362No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT23080.8797877531105147No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC21970.8374756038058062No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC20780.7921139302268846No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA15990.6095236643083679No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC15180.578647231031959No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA14660.558825323249573No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA14000.5336667479873141No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT13870.5287112710417174No Hit
CATAATCACCCACAACAAATTTCCCACCACTAAAATAAACCACCCCCTAA12000.45742864113198345No Hit
ACTCCCTTTTCCCTTTAACTTTATTTTATCCCTCTAAAAATTTCTAAAAT11240.42845816052695784No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC11090.42274030251280803No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9590.36556172237131007No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA8930.340403147109051No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA8370.31905647718955843No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACCC8260.31486338131251523No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC7260.2767443278848499No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAACCCAATTCACCCAT7090.27026408880214686No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA6640.2531105147596975No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA6070.23138265430592825No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5970.22757074896316173No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT4490.1711545498902171No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3720.14180287875091485No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA3630.13837216394242496No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3600.13722859233959503No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC3380.12884240058550867No Hit
CACTCCATTATTCTTATCTTTAATCTCTTTTTATTTATAAAAATCCAATT3300.12579287631129543No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3220.12274335203708221No Hit
CAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT3110.11855025616003903No Hit
CAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA3110.11855025616003903No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA2650.10101549158331301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)