FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324811
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT109893.383198229124014No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC96272.963877454889151No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT92122.836110845999674No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA80642.48267453996324No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC76872.3666070422491847No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC74332.2884077201818904No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA74112.2816345505540148No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT73922.2757849949663034No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA69112.127698877193198No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC67462.076900104984129No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA65502.0165573210266894No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA63611.9583696364963008No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC60851.8733971448011304No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA54051.6640446290304207No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA52131.6049333304598674No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT51771.5938499619778885No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC48801.502412172001564No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT48001.4777824642638335No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA46111.4195947797334452No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC42921.3213838201292443No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT42481.3078374808734927No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC41291.2712007906136185No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT40691.2527285098103205No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT40441.2450317261422796No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC38351.1806866146774586No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA37301.1483601232716873No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA37041.1403554682569248No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA36101.1114155616650914No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC33981.0461468361601054No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA33761.0393736665322295No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT30930.9522460754100077No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG30920.951938204063286No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT29400.905141759361598No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC27800.8558823438861369No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC27260.8392572911631688No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC27250.8389494198164472No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA24630.7582871269753796No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA22510.6930184014703936No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA21680.667465079692498No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC19360.5960389272530795No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19070.5871106581981521No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17750.5464716404308967No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT16680.5135294063316821No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA16360.50367752323659No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC13740.4230152303955223No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12900.3971540372709052No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC11610.3574386335438147No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC10910.33588763927330045No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA9880.30417689056097236No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCACCAGACCCCCGCAAAAC9560.29432500746588014No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9450.29093842265194225No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA9080.2795471828232418No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA9080.2795471828232418No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCACAAAAC9010.27739208339619037No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC8800.2709267851150361No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAGCACC8430.25953554528633577No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA8110.24968366219124352No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7910.24352623525681089No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG7750.23860029370926478No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA7440.22905628196089417No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7410.2281326679207293No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC7020.21612568539858562No Hit
AAAAATCCTAAAAACCCCAAAACTACGGACTAACGAAAAAAATAATCACA6550.2016557321026689No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA6510.2004242467157824No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6190.1905723636206902No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5880.18102835187231958No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5790.17825750975182492No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCACAACCACACTACCTT5620.17302369685755714No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCCACTTCTACCTCCGCCT5590.17210008281739228No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5280.16255607106902167No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC5220.16070884298869187No Hit
CCTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC4330.13330829313046663No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA3970.12222492464848789No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA3610.11114155616650913No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3310.10190541576486017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)