FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281986
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT121504.308724546608697No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT110743.927145319271169No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC110293.9111870802096558No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA97483.4569092082585664No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA88563.140581447305894No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC88473.1373897994935915No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG79952.8352471399289327No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA78742.792337208230196No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT74162.6299177973374563No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA70972.5167916137680595No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC66572.3607554984999255No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA62852.2288340555914123No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA61992.1983360876071862No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA60882.1589724312554526No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA58472.0735071953926787No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC58372.069960920045676No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC54461.9313015539778573No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA53551.8990304483201295No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC47531.6855446724305463No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA46781.6589476073280234No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA43951.5585880150078373No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC41251.4628385806387552No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC38841.377373344775982No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT36201.2837516756151015No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT32051.136581248714475No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC31971.1337442284368728No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC31181.1057286531955488No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC27140.9624591291766257No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC22610.8018128559573879No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT20390.7230855432539204No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT18790.6663451377018718No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA15980.5666948004510862No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13930.4939961558375238No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC12360.4383196328895761No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC12220.43335484740377184No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC10970.38902640556623375No Hit
CACCCAAAATAAACTATCCCCATCACACAATGTAGAACCATGTCGTCAGT8990.31881015369557353No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA8400.29788712914825555No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA7730.2741270843233352No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT7670.27199931911513336No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT7360.26100586553942395No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC6880.24398374387380933No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT6530.2315717801592987No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA6310.22376997439589197No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT6170.21880518891008774No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA5890.2088756179384792No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTATCCTCCCCTATAACT5840.20710248026497768No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA5830.20674785273027738No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA5190.1840516905094579No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACCTCTACCTCCACCT4770.16915733405204514No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4420.1567453703375345No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA4270.15142595731702993No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA4050.1436241515536232No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3910.13865936606781898No Hit
CCTAAAAAAACAACACTCCAAACCCCACTCTATCTTACAACCTAAATGTA3690.13085756030441228No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA3580.12695665742270892No Hit
CACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA3020.1070975154794919No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC3000.10638826041009129No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT2950.10461512273658975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)