FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312349
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT117913.774944052966393No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT105983.3929994973571227No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC105343.3725095966370953No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT100953.2319616838856535No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT95213.0481928868029033No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA81932.623027446862324No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC78522.5138546945884253No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA76852.4603888598971024No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA76092.436057102792069No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA75452.415567202072041No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA74192.375227710029486No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC73162.3422517760581916No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT72922.334568063288181No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC65172.086448171756593No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC61461.9676707785201808No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA57831.8514546228737727No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA57391.8373678161287532No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT56241.8005500257724534No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC53621.7166694946998389No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG52821.691057118799804No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC48961.5674774050821356No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA48121.540584410387099No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC44561.4266093376319438No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC43541.3939535583593992No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA43031.377625668723127No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC38821.2428405405491934No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC36591.1714460427278461No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC35481.1359088711665477No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC35271.1291856224927885No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT30720.98351523456134No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA29710.951179609987546No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC28640.9169230572212493No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA23920.7658100394110434No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT20800.6659217734009073No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC19730.6316652206346107No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19220.6153373309983384No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC19170.6137365575045862No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA14110.4517382799368655No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC12380.39635151705304006No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA12200.39058873247553216No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12170.3896282683792809No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT12130.38834764958427914No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA11370.364015892479246No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAGCCAAATAAATAACT9770.3127911406791762No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8960.2868586100803908No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA8490.2718113392391204No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA7820.2503609744228411No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA7680.245878808640335No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT7030.22506875322155667No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6930.22186720623405232No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC5740.18376879708275037No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5160.16519982455522508No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA4900.15687580238771373No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4820.15431456479771027No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA4250.1360657469689354No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4180.13382466407768231No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3590.11493553685140659No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3260.1043704317926422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)