FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367452
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT128153.4875303440993655No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT120503.2793398865702184No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC102842.7987328957251556No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC98372.6770843538747915No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA88412.4060285425035106No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA75942.066664489511555No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC73962.0127799005040115No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC71701.951275268606512No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT71621.9490981134950962No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA70431.9167129312127842No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA67771.8443225237582053No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT67541.8380632028128845No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA66351.805678020530573No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG65771.7898936459728072No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA65241.775469993359677No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA60951.6587200505099986No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC59761.6263348682276868No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC56831.5465965622720792No Hit
CATAATCACCCACAACAAATTTCCCGCCGCCGAAATAAACCACCCCCTAA56111.5270021662693358No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA53081.4445424164244582No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA52051.4165115443649783No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC50811.3827656401380317No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC50331.3697027094695362No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT49991.3604498002460184No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA49231.3397668266875673No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT48931.3316024950197576No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT45181.2295483491721368No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC44851.220567584337546No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT42061.1446392998269161No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC39221.0673502933716514No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC36991.0066620946409328No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC36961.0058456614741518No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA36480.9927827308056563No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA35310.9609418373011985No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA34710.9446131739655792No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA33180.9029750824597499No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC32990.897804339070137No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC30020.8169774555588212No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT29100.7919401717775382No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT24660.6711080630939551No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA21880.5954519229722521No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT21180.5764018157473629No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC17850.4857777342346756No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17290.47053764845476415No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA16860.4588354397309037No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA15730.4280831237821538No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC15630.425361679892884No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC14860.4044065619455058No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC14090.38345144399812764No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA13980.3804578557199308No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC13280.36140774849504154No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT11590.3154153467663804No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10380.2824858757062147No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10230.27840370987230983No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA9710.2642522016481064No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA8930.24302493931180127No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8530.2321391637547217No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA8400.22860128669867086No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG7450.2027475697506069No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7220.19648824880528615No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACGACACAA5890.16029304507799658No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACGACACA5670.15430586852160283No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5440.14804654757628208No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5330.1450529592980852No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA5220.1420593710198883No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA4850.1319900286295897No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4810.13090145107388176No Hit
CCCCTAATAAAAACTTCCTTCCACTAAGCACAAACATTAAAACCTTCATC4490.1221928306282181No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4260.11593350968289735No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC4230.1151170765161164No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4000.10885775557079565No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA3850.1047755897368908No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA3740.10178200145869393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)