FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337693
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT158164.683543928953221No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT110913.2843440639871124No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA107783.191656326900469No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC93372.764937383955249No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC84272.495461854406221No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA82902.4548924614960925No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC79912.3663505017871262No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG78362.3204508236771266No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA74852.2165102622796446No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT74362.2020000414577736No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA72502.1469204277257745No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT71882.1285605564817747No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA70972.101613003526872No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA68112.0169206942400346No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT65281.9331167658198423No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC62151.8404290287331986No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC61851.8315452200667472No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC59781.7702469402682317No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA55851.6538690467377173No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA54961.6275137476939112No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT52251.5472633427402995No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC51601.5280150906296548No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC47831.4163752283879143No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT44681.3230952373901739No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT40821.2087902325484983No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC40471.1984257891043049No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA38291.1338701127947575No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT36701.0867859268625646No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC35481.0506584382856619No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA33650.9964672054203079No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA32220.9541210507768891No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC27250.806945953869343No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT23680.7012286307385702No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA23180.6864222829611512No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA21010.6221627336071521No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC17690.523848584365089No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT15550.46047741587773516No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC14630.4332337359672839No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC14520.4299763394562517No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC12520.3707509483465751No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAACGACACAA12150.359794250991285No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA12130.35920199708018824No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAGCCTTCATC10030.29701533641502786No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT9370.2774709573488346No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9320.2759903225710927No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC8650.25614981654935104No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAACGACACA8370.2478582617939963No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7900.23394029488322232No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC7540.22327972448348055No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6970.20640048801722274No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC6310.1868561089510295No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC5930.17560328464019095No Hit
AACTTACACATCTCAAAAAAACCCAAAAACCCCGCAACCACGCTACCTTA5620.16642334901819109No Hit
AACCTCCTCTCCAAAACTAAATCTTACCCACCTAAAACCTAATCACCAAC5440.16109306381832017No Hit
TAAAACTAAACCCCACATACCTTCCCCTCTACCAATCCCAAAATACCTAT5410.16020468295167503No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5300.15694728644064282No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA5060.14984023950748165No Hit
CCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA5060.14984023950748165No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC4860.143917700396514No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAACGACACAAA4680.1385874151966431No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4290.1270384639302562No Hit
TCTTTCCTCTTACCCAACACTTTATAAGTAAATAATCACCAAAACCACAC4280.1267423369747078No Hit
CCTAAAAAAACAACACTCCAAACCCCACTCTATCTTACAACCTAAATGTA4230.1252617021969659No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA4030.11933916308599823No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4000.11845078221935307No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3820.11312049701948218No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC3770.11163986224174027No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA3630.10749408486406292No Hit
GCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA3470.1027560535752888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)