FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316583
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT129924.103821114841921No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT122523.8700751461701985No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT107693.4016355900348403No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC94122.972996023159803No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC92742.9294055587318333No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC92542.923088100119084No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC92072.908242072379123No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT90202.8491738343499176No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA88072.781892900124138No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA87092.7509373529216665No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC86632.7364071981123432No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC81832.584788191406361No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA78942.493500914452134No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC76022.401266018705995No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT74912.3662041234052364No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT73032.3068200124453933No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT68432.1615184643521603No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC63221.996948667490042No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA61861.9539899489233472No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT57291.8096360196220265No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT56301.7783645994889175No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC56061.7707836491536184No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA55461.7518312733153705No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC45841.4479615140421311No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA38821.226218716734632No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT26740.8446442165245766No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC22630.7148204420325791No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC21450.6775474362173585No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC20060.6336410988587511No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA15430.48739193197360564No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA14210.44885543443583514No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA13950.4406427382392611No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC11070.3496713342156717No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT10850.34272212974164756No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA10810.34145863801909765No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG9980.31524118477618823No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA8960.283022145851167No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8430.26628088052738147No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAACAACATTT8240.2602792948452697No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC7730.24416977538275902No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCTGTTAACTCC7570.23911580849255962No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC7050.2226904160994115No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA7010.22142692437686168No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA6760.2135301011109251No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA6450.20373804026116374No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6410.2024745485386139No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA6370.20121105681606402No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5500.17373011185060475No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT5260.16614916151530562No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4670.1475126586076953No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4420.1396158353417587No Hit
AAAACAAAAAAAACTCCTAATCCCTAAACAATCTGTAGAACCATGTCGTC4300.13582536017410918No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC4200.13266663086773453No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA4080.12887615570008498No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4060.12824440983881003No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT3980.12571742639371034No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3940.12445393467116048No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC3780.11939996778096108No Hit
AATATTTAAAAAACCCTACCTTAAAAACCACATTCACTTACCAATCCACT3720.11750473019713628No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3540.11181901744566196No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3420.10802854227801241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)