FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325739
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC152444.679820346964901No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT151074.637762134715217No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT142724.381421935967139No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT112303.4475454274741435No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT98713.0303402417272722No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA98043.0097716269774266No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC97622.9968778684775232No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC95422.92933913347803No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC88722.7236529859795726No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA79312.4347713967317395No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC75192.3082897657326877No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA74882.298772943982759No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA73852.267152536232996No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA62261.9113462004856647No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT59331.8213968852363396No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA58811.805433184236459No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA58311.7900834717365743No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA58121.7842505809866183No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA56531.7354384952369843No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT53841.6528570419876036No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC51661.5859322954881054No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC50521.5509349509883679No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC47531.4591436702390563No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC45761.404805687989464No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA45361.3925259179895562No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC45001.381474124989639No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA38531.1828488452411288No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC33141.0173789444923695No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC28880.8865993939933505No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC23430.7192875277446054No Hit
CATAATCACCCACAACAAATTTCCATAATCACCCACAACAAATTTCCATA23320.7159105909946306No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC23300.7152966024946352No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC20930.642538965245181No Hit
CATAATCACCCACAACAAATTTCCATAATCACCCAATCTAAAACCAAAAA20330.6241193102453191No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA17510.5375469317459685No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA16750.5142153687461434No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC16460.5053125354962101No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACAATCAAACA14530.44606264524665457No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA9860.3026963304977298No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT7950.24406042874816955No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA7250.22257083124833074No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCACCACCCGCAAA6500.1995462624985034No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5920.18174059599863696No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA5560.17068880299871983No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5250.16117198124879123No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4800.14735723999889483No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA4760.14612926299890402No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA4250.13047255624902146No Hit
CCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA4060.1246396654990652No Hit
AATACCTTAACTCCCTAAAACCCAATATAAATCAAAAATATCCCAAAACT3890.11942076324910433No Hit
CATAACACAAACACAAAAAACCAAAAAAAAATCCTATAACTCTAAAAAAA3750.11512284374913659No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3560.10928995299918033No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT3530.10836897024918722No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3470.10652700474920104No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3370.10345706224922407No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAATAATCACAC3370.10345706224922407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)