FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258701
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA111394.305742923297553No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC102943.9791110200579043No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA100553.886726375236277No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA96473.729015349766719No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA93103.5987491351019125No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAACA86383.3389897990344064No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT84593.269797952075949No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG83903.2431262345333027No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA79843.086188302325851No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC79043.055264571841624No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA76572.9597875539715734No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT71802.7754048109593703No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT59112.28487713615332No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA58802.272894190590682No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA57782.233466434223293No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA50541.9536066733410387No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT49821.9257753159052342No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATACCCT49751.9230694894878644No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA46451.7955091012404283No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC45801.7703835702219937No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT42931.6594446871098296No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT42311.6354787959845536No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA41661.6103532649661194No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA40521.5662869490260956No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC34071.3169643719970159No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT32691.2636209369117242No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA23570.911090409391537No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTAGCTATATTCCCACA14140.5465769363087115No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA14000.5411652834739719No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT11600.448394092021291No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT10590.4093528822849545No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA10500.4058739626054789No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA6370.2462302039806572No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAACA6240.2412050977769703No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5450.21066791392379622No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAACTTCGATT5420.20950827403063768No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT4170.16118994514903306No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAACAACTT3660.14147606696533838No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA3360.12987966803375325No Hit
AACTTCTCCTATCCCCTCCCTACTACCACCGAACCCCTAATTCTTAACAA3290.1271738416163834No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3260.12601420172322486No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3190.12330837530585502No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3150.12176218878164367No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3090.11944290899532665No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC3080.1190563623642738No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATACCCT2930.11325816289848124No Hit
AACTTCTCCTATCCCCTCCCTACACTTCCTCCCAAATACATTCAAATTCC2860.1105523364811114No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2830.10939269658795289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)