FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190355
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA110495.804418060991305No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA106595.599537705865357No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA86474.542565207113026No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT82084.311943474035354No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA72183.7918625725617923No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT69203.635312967875811No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT66673.5024034041659005No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT64753.4015392293346642No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG63353.3279924351868875No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCAAATATCTA58603.07845866932836No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA57653.0285519161566543No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC55282.9040477003493472No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA51242.6918126658086208No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA46832.4601402642431247No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT45892.410758845315332No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA43522.2862546295080244No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA38662.0309421869664575No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT36811.9337553518426098No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC33051.7362296761314386No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA30711.6133014630558693No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT28411.4924745869559508No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA25891.360090357489953No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC23561.2376874786582963No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCGAATATCTA13610.7149799059651704No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC10770.5657849806939665No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA8070.42394473483754036No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA7630.40083002810538204No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7520.39505135142234243No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA7260.38139266108061254No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4640.24375508917548788No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA4400.23114706732158338No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2990.15707493892989413No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2890.15182159649076724No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2770.14551758556381497No Hit
CACAAAACACCTAAATTATACCCAAAAAAAACTAAACCCGCCGAATATCT2660.1397389088807754No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2230.11714953639252974No Hit
CACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT1910.1003388405873237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)