FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350689
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG154454.404187185797102No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG152534.34943782097528No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG151764.327481044458195No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT147624.209427726561141No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC147204.197451303006368No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG132663.782838925657776No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT129503.6927305960551946No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT113603.2393374186244794No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT112133.1974199361827713No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG111213.171185865538982No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT108723.100182783035681No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG97212.7719717470465284No Hit
TTGTGGGGATTTAGGTTGTATTATGTGGATGAGGTTTTTAGGTAGTAGGT92302.6319616526323895No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA90642.5846262642968556No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT85392.4349209698621856No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG75262.1460610398387177No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA59451.6952342388840254No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA55381.5791769915794336No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG54931.566345109199319No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT44321.2637978379703954No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG42791.220169437878006No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA39861.136619625936371No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG27340.7796081428274055No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG26680.7607880486699041No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT25480.7265696956562653No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTCAGGGGGCG22500.6415941190057287No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG20230.5768644012215952No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT19600.5588997658894348No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT17640.5030097893004913No Hit
GGGGATTGGGATAAAGGAGTATTATTTTTGGTTTTTGTTTGAGTGATTTC17470.4981621892902259No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT14880.42430757736912195No Hit
TGGGGAGGGAAGAGTAGATTTTATGATATTTTTGGGGTTTTTAGATAGTT13620.3883783067048011No Hit
GGAGGAGGGTTTAGTAGGTGGTTTAGGATGGAATTTTTTTGGAAGTGTTT13360.38096433021851267No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG10380.29598875356797616No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG10260.2925669182666123No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT9960.2840123300132026No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT9340.2663328476228225No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT9230.26319616526323897No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG8440.24066908286259334No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA8390.23924331815369174No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT7890.22498567106467557No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTT5800.16538870623258786No Hit
TGTTTGTTTTGGGATTTTTTTTTATTGTTTAGTTTTATTGGGGTTTAGTA5770.16453324740724687No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT5300.15113105914357164No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTAG4970.14172101206482096No Hit
GTTTAGGATTGGATTGAGGATTAAACTTTTTAAGGTATTTTTTTAAAGAG4290.12233061202375894No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG4210.12004938848951636No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT4080.11634240024637214No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTAAAGAGT3780.10778781199296242No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTGAGGAAATTAAGG3670.10465112963337886No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGCTGAGTGTTAGGTT3510.10008868256489369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)