FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350689
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA154324.400480197553958No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG148704.240224244273416No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA140213.9981293967019207No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT127923.647676431253903No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA127543.6368406194662506No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT123153.511658478024689No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA122013.4791510426617314No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC112753.2150994185731516No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA110183.1418151125356086No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA108153.083929065354203No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT100732.8723455825532023No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT97712.7862293941355443No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT94392.691558617464477No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC93902.6775861233172416No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA93812.6750197468412185No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA93122.655344193858376No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA68451.9518718864863167No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA62331.7773582861167587No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT61921.7656670155037655No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC53911.5372595091377261No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA53151.5155878855624214No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA47671.359324073466804No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA42691.2173179084602026No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC35091.0006016727071565No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA28010.7987133899266872No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC24590.7011910838378165No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT20330.5797159306393984No Hit
CTCCAAACTACAAAAAACTCCTTCAAACCACCTATCCACCTCCTAAAACA19480.5554779305880709No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA17740.5058613187182945No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA15620.4454088950608659No Hit
AACCCAATCTAAAACCAAAAACCTATCTCCACATTAATATCATTAACAAC12940.3689879066637391No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT11990.34189837719460836No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA11460.3267852712802512No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11350.3236485889206676No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGGA8080.23040357695850172No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7710.2198529181126297No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT7240.2064507298489545No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA7220.20588042396539383No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT6360.181357270972286No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5730.1633926356401256No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA4940.14086555323948No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT4250.12119000025663765No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3900.11120964729432631No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAATGAAAAACCCAAATATCCT3520.100373835506674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)