FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268193
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA134465.013553672168923No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA113204.220840961546349No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA109074.066847382295586No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC105003.9150909979007658No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG97953.6522206023274286No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT95713.5686986610388787No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT87223.2521355889229024No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA82583.079125853396621No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC80943.0179758606675042No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA80503.0015697650572535No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA71482.6652448050471116No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT69102.5765027424280276No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA66402.475828973910579No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA64782.4154247128001103No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT63882.381866789960961No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC59002.1999082750109067No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT55392.065303717844985No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA46831.7461305850637414No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT46351.7282330262161951No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA38821.4474650717953115No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA34551.2882513712140138No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA29401.0962254794122144No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC27061.0089748800304257No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA24370.9086739773223015No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT23460.8747431886738282No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT22350.8333550838388772No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA21200.7904755157666308No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA13450.5015045135406219No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA11170.41649110901477665No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA10820.40344080568844076No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA10090.3762216016077974No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT9970.37174721189591076No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACATCTAAAA9380.3497481291458017No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT8730.3255118515397494No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8460.3154444746880045No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAACTTCAATT8450.31507160887868063No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA7880.29381825774721937No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA7860.2930725261285716No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA6740.25131155548429673No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAACCTTACACACCCCCAAAATTA6500.24236277606052356No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6380.23788838634863696No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCGATATAAAAAAAACAACT5900.21999082750109064No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC5330.19873747636962932No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCATAAAACA4760.17748412523816803No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4470.16667101676777546No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3670.13684175202186485No Hit
CCTAAAACAAACTAATACCACCCACTAAGCGAAAACGAAACCCTAAAAAA3660.13646888621254097No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA3510.13087589907268274No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3470.12938443583538722No Hit
AATCTAAACACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3050.11372407184378414No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC2870.10701248727595426No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2860.10663962146663036No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2770.10328382918271543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)