FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224249
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA127295.676279492885141No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC116395.190212665385353No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG115895.167916021922061No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA105394.699686509192906No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT99924.45576122970448No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA96574.306373718500417No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA93944.1890933738834955No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTG83873.740038974532774No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA83273.7132830023768224No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA83103.7057021435993027No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA79383.5398151162324023No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA65842.936022011246427No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA62752.7982287546432763No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT61702.7514058033703606No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA50482.251069124054065No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT47232.10614094154266No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA40031.7850692756712405No Hit
AACCCAATCTAAAACCAAAAACCTATAACCTCCTCTCATTCTCTATTAAT33131.4773755958777965No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA30011.338244540666848No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC27701.2352340478664343No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT22481.0024570901096548No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC18020.803571030417081No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA13370.5962122462084557No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA10550.47045917707548307No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA8880.39598838790808427No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA6530.2911941636306071No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT6290.2804917747682264No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4240.18907553656872494No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA3370.15027937694259505No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3190.14225258529580956No Hit
AACTTCTCCTATCCCCTCCCTACCGTACCACACTCCTAAAAACCTAACTT3100.1382391894724168No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC2390.10657795575454072No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2350.10479422427747728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)