FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262921
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA113804.328296332358389No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT101423.8574324607011232No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA101263.851346982553695No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT98103.7311587891419853No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG92413.5147439725240663No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC90593.445521658597069No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA88733.374777975133215No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT87633.332940312869645No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA87023.3097394274325747No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA83093.1602648704363667No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA77322.9408073147447333No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA76822.9217901955340198No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT75072.8552302782965224No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT57182.1747977529371942No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA55282.1025326999364826No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA54472.071724966815127No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA50381.9161649316714908No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC50011.9020922634555626No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC48781.8553101501972074No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT42301.6088482852263608No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA37691.4335104461035824No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC37071.4099292182822976No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC36051.3711342950924421No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA33991.2927837639443027No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA24600.9356422651671035No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA23900.9090182982721046No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT23830.9063559015826046No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA19800.7530779207442541No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA15600.5933341193742607No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8700.3308978742664146No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT8410.3198679451242008No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAACATCTA7930.3016115106819159No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA7610.28944055438705923No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT7500.28525678816070227No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT7140.27156446232898857No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA6870.2612952179552033No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC6790.2582524788814891No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA6150.23391056629177587No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAGACTACCTACCG6030.22934645768120462No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5500.20918831131784832No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA5320.2023421484019915No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5070.19283358879663473No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC4990.18979084972292057No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA4720.1795216053491353No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCAAAATTAA4360.16582927951742157No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT4350.1654489371332073No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3890.1479531874593509No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3820.14529079076985102No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAAGTACCTACCG3720.14148736692770833No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCTAAAAAA3550.13502154639606573No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3430.1304574377854945No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3300.125512986790709No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAAGCGTCCTAACCT2860.10877792188528114No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAACAACTT2810.10687620996420978No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA2690.10231210135363854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)