FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248744
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC121384.879715691634773No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT115674.650162415977873No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG105524.24211237255974No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT101104.0644196442929275No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG99884.01537323513331No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG98683.967130865468112No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT97373.9144662785836037No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG96813.8919531727398446No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT81623.281285176727881No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT75813.0477117035988806No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG72172.901376515614447No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG70732.8434856720162096No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG64932.6103142186344193No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA60352.4261891744122472No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA53722.1596500820120283No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA53712.1592480622648185No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG51252.060351204451163No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT39851.602048692631782No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT29841.199626925674589No Hit
GGAGGAGGGTTTAGTAGGTGGGCGTTGAATAGGGTTAGTTTTTGTTTAGG29831.199224905927379No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG28791.157414852217541No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT23700.9527868008876595No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTGTATAGGGTGGAAGAATTTAA22770.9153989643971312No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG18190.731273920174959No Hit
GGGGATTGGGATAAAGGAGTATTATTTTTGGTTTTTGTTTGAGTGATTTC17860.7180072685170296No Hit
TTGTGGGGATTTAGGTTGTATTATGTGGATGAGGTTTTTAGGTAGTAGGT16770.6741871160711413No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGCGGTGG16250.6532820892162223No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG15640.6287588846364134No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT13820.5555912906441964No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA13810.5551892708969864No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT11930.47960955842150965No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT11260.45267423535844076No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT9460.3803106808606439No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGTGTT9410.37830058212459394No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT9250.37186826616923424No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTC8460.34010870613964556No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG7010.281815842794198No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT6530.26251889492811886No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA6410.25769465796159907No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGATTTGGGTTTAGGACGGTGAGG5640.22673913742643037No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT5600.22513105843759046No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG5090.20462805132988132No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGCGG4550.18291898498054224No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG3170.1274402598655646No Hit
TGTTTGTTTTGGGATTTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGT3050.12261602289904479No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGTGGAGAGGGGTTT2940.11819380567973498No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTAAAGAGT2680.10774129225227544No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG2660.10693725275785547No Hit
GGGGATTGGGATAAAGGAGTATTATCTTCGGTTTTTGTTTGAGTGATTTC2610.10492715402180555No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGTGG2520.10130897629691571No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTTGTA2490.10010291705528575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)