FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248744
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA100744.049946933393368No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA100434.037484321229859No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT93653.7649149326214904No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC89543.599684816518187No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT83673.363699224905927No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA80583.239475123018043No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG79863.210529701218924No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA73162.941176470588235No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA72242.904190653844917No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA67822.7264979255781046No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT67522.714437333161805No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA60162.4185507992152573No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA58562.3542276396616604No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC54182.1781429903836873No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC50912.0466825330460234No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT50492.029797703663204No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA48141.935323063068858No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA44901.8050686649728236No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA40341.6217476602450713No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA38791.5594345994275238No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT35961.4456630109670987No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT35541.4287781815842793No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA33281.3379217187148233No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA27491.105152285080243No Hit
AACCCAATCTAAAACCAAAAACCTAAATTCTAATTTTACCTTTAAAAAAA27241.0951017913999934No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT27171.0922876531695236No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC26311.0577139549094652No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC24470.9837423214228282No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA19830.7972051587173962No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA15410.6195124304505837No Hit
ACCAAACAACCAAACTAAACAACGCCAAAAACGAAAAACCCAAATATCCT13960.5612195671051362No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGGAACCCATTCGAA13830.5559933103914064No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT12620.5073489209789984No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT12180.4896600521017592No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACCAAACTACCTACCG10350.41609043836233234No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9940.399607628726723No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCA9870.3967934904962531No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA7700.30955520535168685No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA7600.30553500787958704No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAACCCTAAAAAC6060.2436239668092497No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA5500.22111086096549062No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA5450.2191007622294407No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA5370.21588460425176087No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5370.21588460425176087No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT4960.19940179461615154No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3780.15196346444537356No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3510.141108931270704No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3380.13588267455697425No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT3250.13065641784324447No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2840.11417360820763515No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2680.10774129225227544No Hit
CACAAAACACCTAAATTATACCCAAAGAAACTAATCCCGCCGAATATCTA2520.10130897629691571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)