FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201896
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA154767.665332646511075No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA148357.347842453540436No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC144477.155664302413125No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA137716.82083845147997No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC135816.726730593969172No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAGAACCCAAATATCCT128356.357233427110988No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTGAACAAAACGTCTAAAA113835.638051273923208No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAGACTCCCCACAAAAACAAA73483.639497563101795No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA62823.1115029520148987No Hit
CCAAAACTCAACAACCTAAACCCTACCTACTCGCTTCCCCAAAACAAACC54922.7202123865752665No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA54092.6791021119784446No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT46252.2907833736181002No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC37031.8341126124341245No Hit
AACTTCTCCTATCCCCTCCCTACTCCCTCTCAAACCTAACCTAAAACCAA27571.3655545429329952No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA16160.8004120933549945No Hit
AACTTCTCCTATCCCCTCCCTACCCCAATTAAATAAACATAAAACTTCTA14370.711752585489559No Hit
CCAAAACTCAACAACCTAAACCCCACCATACGGTAGCAGAGACTTGGTAT12060.5973372429369577No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAGAACCCAAATATCCT11460.5676189721440742No Hit
AACTTCTCCTATCCCCTCCCTACAACCCAAAACCAAAAACTACATAACAA9760.483417204897571No Hit
AACCCAATCTAAAACCAAAAACCTACGCTCGCTATACGAAATCTAAAAAC8650.4284384039307366No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTGAACAAAACGTCTAAAA7870.38980465189998814No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC6600.3269009787217181No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTGAACAAAACTTCTAAAA6220.30807940721955857No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC5840.28925783571739905No Hit
AACCCAATCTAAAACCAAAAACCTACCCACCTACTAAACCCTCCTCCTGT4140.2050560684708959No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTGAACAAAACGTCTAAA3650.18078614732337442No Hit
CACAAAACACCTAAATTATACCCAAAAAACTAATCCCGCCGAATATCTAA3510.17385188413836827No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAAACAAC3450.1708800570590799No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2910.1441336133454848No Hit
CCAAAACTCAACAACCTAAACCCTACCTACTCGCTTCCCCAATACAAACC2850.14116178626619647No Hit
CACAAAACACCTAAATTATACCCAAGAAAACTAATCCCGCCGAATATCTA2710.13422752308119032No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2650.13125569600190196No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTGAACAAAACGTCTAAAAA2590.12828386892261362No Hit
AACTTCTCCTATCCCCTCCCTACACTTCCTCCCAAATACATTCAAATTCC2490.12333082379046639No Hit
CACAAAACACCTAAATTATACCCAAAAAAAACTAATCCCGCCGAATATCT2270.11243412449974244No Hit
AATCTAAAAACCTCCTCTCCAAAACCTTATACAACCCCTCCTACCTGTAG2210.1094622974204541No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTGAACAAAACGTCTAAAAA2060.10203272972223323No Hit
CACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT2030.10054681618258907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)