FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230797
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA93594.055078705529101No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT83633.6235306351469045No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA80763.499178932135166No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA80713.4970125261593523No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC79463.4428523767639962No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA76933.333232234387795No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA75673.2786388037972762No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT72613.1460547580774447No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT70323.0468333643851526No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG69643.0173702431140788No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC66342.8743874487103382No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA63542.753068714064741No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT48802.114412232394702No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC47652.064584894950974No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA45851.9865942798216616No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA44201.9151028826197913No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA43421.8813069493970893No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT40671.7621546207273058No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA39441.7088610337222754No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA38771.6798311936463646No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA37401.6204716699090542No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT36851.5966412041750977No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC31991.3860665433259531No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA30201.3085092093918034No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA29681.285978587243335No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT18550.8037366170270843No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT18360.7955042743189903No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCGAATATCTA15790.6841510071621382No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC13590.5888291442263115No Hit
ACCAAACAACCAAACTAAACAACGATAAAAACGAAAAACCCAAATATCCT11710.5073722795356959No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11510.4987066556324389No Hit
CCAAAACTCAACAACCTAAACCCCGAAAACAACTACTAAACTACCTACCG11090.4805088454355993No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA10140.4393471318951286No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT9980.43241463277252307No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT9120.39515244998851806No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC9010.39038635684172673No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA7690.3331932390802307No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA7470.32366105278664803No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA7110.3080629297607855No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCACCAAATATCTA6540.2833659016365031No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACGTATCCACCTCCTAAAACA6210.26906762219612906No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCCGAAAAA6190.26820105980580333No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA4870.21100794204430734No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4490.19454325662811908No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3990.17287919686997663No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT3930.17027950969899955No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAGACGAAACCCCGAAAAA3920.1698462285038367No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3850.16681326013769676No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA3650.15814763623443978No Hit
ACCAAACAACCAAACTAAACAACGATAAAAACGAGAAACCCAAATATCCT3510.1520816995021599No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2700.11698592269396915No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2690.11655264149880631No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA2620.11351967313266637No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2520.10918686118103788No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2440.1057206116197351No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2420.10485404922940939No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2370.10268764325359514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)