FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263992
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA122054.623246158974514No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT105173.983832843419498No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA102293.874738628443286No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA100643.8122367344464982No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT98443.728900875784115No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC93903.556925967453559No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA90723.4364677717506593No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA90483.4273765871693085No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG89293.3822994636201096No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA85173.2262341283069187No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA72672.7527349313615566No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT71172.695915027728113No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC70902.685687445074093No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC61272.3209036637473863No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT61062.3129488772387043No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA58702.2235522288554197No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA50411.9095275614412557No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA48721.845510470014243No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT46981.7795993817994484No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA40301.526561410951847No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT38351.4526955362283707No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA32951.248143883147974No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT31401.1894299827267494No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA29561.119730900936392No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC25510.9663171611260948No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA23930.906466862632201No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA19300.7310827600836389No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA17150.6496408982090366No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC12650.47918118730870635No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT11570.43827085669262705No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11430.432967665686839No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC9200.34849540895178643No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA8440.3197066577775084No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7410.28069032394921056No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA5350.20265765629261492No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT5110.19356647171126398No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4910.18599048456013817No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT4160.15758053274341646No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAACAACTT3340.12651898542380072No Hit
CACAAAACACCTAAATTATACCCAAAGAAACTAATCCCGCCGAATATCTA3140.11894299827267492No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3110.11780660020000606No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2980.11288220855177429No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2880.10909421497621141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)