FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190399
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA105205.52523910314655No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA81034.255799662813355No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT75433.961680471010877No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG72993.8335285374397974No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC67733.55726658228247No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA65963.4643039091591867No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA65823.4569509293641247No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA64223.372916874563417No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT63323.325647718738019No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC61313.220079937394629No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA55112.894447975041886No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC53932.8324728596263635No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT53412.8051617918161336No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA49482.5987531447118943No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA49402.594551441971859No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT49082.5777446310117176No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA40832.144444035945567No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT40272.1150321167653194No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT32101.685933224439204No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA32081.6848827987541952No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCGAATATCTA30341.593495764158425No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA26431.388137542739195No Hit
CCAAAAAAACCAACAAAAAAACTAAATAGAACCGAAAACTTCTCCATCTT19911.04569876942631No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCAAATATCTA16210.8513700176996727No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC15230.7998991591342393No Hit
CTCCAAACTACAAAAAACTCCTTCAAGTCACCTATCCACCTCCTAAGGCA12740.6691211613506374No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA10610.5572508258971949No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT10430.5477969947321152No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA9260.48634709215909744No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8920.46848985551394706No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTACTAATC7940.4170189969485134No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA5910.3104007899201151No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5130.26943418820477No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3470.18224885634903545No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3410.1790975792940089No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT3340.1754210893964779No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA3310.17384545086896466No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2800.147059595901239No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2470.12972757209859295No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2160.11344597398095578No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1970.1034669299733717No Hit
CACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT1960.10294171713086729No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA1940.10189129144585844No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAAATAAAAAACAAACTCTAA1920.10084086576084958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)