FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194437
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT87074.4780571599026935No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG77633.992552857737982No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT76203.9190071848465053No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT74133.8125459660455574No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG71233.663397398643262No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG70783.640253655425665No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA65633.3753863719353827No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG65093.3476138800742654No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT63923.2874401477085127No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG62373.20772280995901No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG56902.9263977535139913No Hit
GGGGATTGGGATAAAGGAGTATTATTTTTGGTTTTTGTTTGAGTGATTTC55592.8590237454805414No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT47732.4547796972798386No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC44652.296373632590505No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT40632.089622859846634No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG37441.92555943570411No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA36981.9019013870816768No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA32731.6833215900265894No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG31731.6318910495430397No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG31401.6149189711834682No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT20781.0687266312481678No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG16910.8696904395768295No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG15180.7807156045402881No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTTATT14930.7678579694194007No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA13970.7184846505551926No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG11660.5996801020381923No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT8390.43150223465698406No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT7510.38624335903146007No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG7170.3687569752670531No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGGTTTC7150.3677283644573821No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT6810.3502419806929751No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT5630.2895539429223862No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT5070.2607528402515982No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT5070.2607528402515982No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGTGTTTTTAGATAGTT5070.2607528402515982No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTCAAGGCACTTTTCCAAAGAG4780.2458379835113687No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT4700.24172354027268472No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG4450.22886590515179722No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGCATCGTCGTTTTTTTTTA4240.21806549165025177No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA4190.21549396462607426No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGCGGTGG4160.21395104841156776No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGATGGTGAGG4090.21035091057771926No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT3670.18875008357462827No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT3560.1830927241214378No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATTGTCGTTTTTTTTTA3550.1825784187166023No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGCTGAGCGTTAGGTT3380.17383522683439878No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGGGG3310.17023508900055032No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT3250.1671492565715373No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATGTTTTTTAGTAAAGTTT3170.1630348133328533No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTATTT2910.14966287280713034No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTT2580.13269079444755885No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTATGTTTTGTTGT2500.12857635120887484No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.12446190797019087No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGTTGAGCGTTAGGTT2310.11880454851700036No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTACGTTTTGTTGT2130.10954705122996139No Hit
TGTTTGTTTTGGGATTTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGT2130.10954705122996139No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTAG2090.10748982961061937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)