FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251426
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA111644.4402726846070015No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT107014.256123073985984No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA105234.1853268953887035No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT94663.764924868549792No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC89113.5441839746088313No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA88123.5048085719058495No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA81793.2530446334110237No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC74152.949177889319323No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA70192.791676278507394No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG69642.769801054783515No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT67422.6815046972071306No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA64652.5713331159068673No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA48931.9460994487443624No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC45271.8005297781454581No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA45131.79496153937938No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA43851.744051927803807No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA42481.6895627341643267No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT41751.6605283463126328No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC41511.6509827941422128No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA41481.6497896001209105No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA37361.485924287862035No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT37151.4775719297129175No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT36831.4648445268190242No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA31971.2715470953680208No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG31371.247683214941971No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT29911.1896144392385832No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA26361.0484198133844551No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA25461.0126239927453804No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA22690.902452411445117No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT22310.8873386205086188No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT19630.780746621272263No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC13770.5476760557778432No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA12210.48562996667011366No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT11570.46017516088232724No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11170.4442659072649607No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAACCTTACACACCCCCAAAATTA10780.4287543849880283No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATGTCTA9870.3925608330085194No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA8070.32096919173037No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA7030.279605132325217No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATCA6360.252957132516128No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6080.2418206549839714No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT6040.24022972962223477No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAACTTCGATT5770.22949098343051236No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA5450.2167635805366191No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA4430.17619498381233445No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTGA3270.1300581483219715No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2680.10659199923635583No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2640.10500107387461917No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAACAACTT2620.10420561119375085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)