FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210464
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA99104.708643758552531No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA97554.634996959099894No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG88064.184088490193097No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA83813.98215371750038No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT82173.9042306522730725No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA80603.8296335715371748No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC74633.5459746084841113No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA71643.403907556636764No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA68873.272293598905276No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC61802.9363691652729207No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA58422.775771628402007No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT58322.7710202219857076No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT56422.6807435000760225No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA53682.550554964269424No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT53502.542002432720085No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT52732.5054166033145813No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT50042.377603770716132No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC48482.303481830621864No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA35671.6948266686939335No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA34081.6192793066747757No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA30451.4468032537631137No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT28281.3436977345294208No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC23921.1365364147787744No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA21891.0400828645279003No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT20830.9897179565151284No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA15370.7302911661851907No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT11160.5302569560589935No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9770.4642124068724342No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACACCCTCTATTAATC9300.4418807967158279No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC7880.3744108256043789No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT7060.33544929299072523No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC6510.3093165577010795No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA5600.2660787593127566No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTGA5410.25705108712178804No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA4920.23376919568192184No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4590.21808955450813441No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3960.1881556940854493No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3860.18340428766915007No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3590.17057549034514216No Hit
CACAAAACACCTAAATTATACCCAAAAAAACCAATCCCACCAAATATCTA3290.1563212710962445No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3120.1482438801885358No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3120.1482438801885358No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATACAAAAAAAACAACT3050.14491789569712635No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2560.12163600425726016No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA2550.12116086361563022No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2430.11545917591607116No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAAACAAC2190.10405580051695301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)