FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170310
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA81774.801244788914333No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG72964.283952791967589No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT65403.8400563677998942No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT61443.607539193235864No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA60983.5805296224531733No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC60893.575245141213082No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA55303.2470201397451706No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA53213.1243027420585987No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC53073.1160824379073455No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA52413.077329575480007No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA49572.9105748341260056No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT47412.783747284363807No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA45502.6715988491574185No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA44722.6258000117432916No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC44102.5893958076448826No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA41912.460806764135987No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT40442.3744935705478247No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT29871.7538606071281782No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA27761.629968880277142No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT26851.5765369032939933No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT21991.291174916329047No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA21051.2359814455992015No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA15520.9112794316246844No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA14930.8766367212729729No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC14430.8472784921613529No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA12390.7274969173859434No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA10290.6041923551171393No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC10080.591861898890259No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA8100.47560331160824376No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA7980.46855733662145505No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7780.456814044976807No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT7140.41923551171393336No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4940.29005930362280546No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4890.28712348071164345No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT4720.27714168281369267No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA3100.1820210204920439No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAAACAAC3080.18084669132757913No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2800.16440608302507193No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2640.15501144970935354No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2590.15207562679819153No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2550.14972696846926192No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2550.14972696846926192No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT2480.14561681639363513No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAACC2330.13680934766014916No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA2300.13504785391345195No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCACAAAAACAAAA2130.12506605601550114No Hit
AACTTCTCCTATCCCCTCCCTACCTAACACTCAAAACCTCCATCTCCACC2040.11978157477540954No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC2040.11978157477540954No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2020.11860724561094475No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2000.11743291644647993No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA1980.11625858728201514No Hit
CACAAAACACCTAAATTATACCCAAAGGAACTAGTCCCGCCGGGTGTCTA1910.11214843520638836No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1900.11156127062415595No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1880.11038694145969115No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACCTCTCCATCTT1860.10921261229522636No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAGAACTTCTCCATCTT1800.10568962480183196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)