FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196198
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA102985.248779294386283No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA78213.9862791669639854No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA77593.954678437089063No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG76513.8996320044037143No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC76373.8924963557222805No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT72363.688110989918348No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC70903.613696367954821No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT69333.5336751648844533No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA69253.529597651352205No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA68743.5036035025841237No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA63613.2421329473287193No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA62043.1621117442583517No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA55182.812464958868082No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT55162.8114455804850205No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA53652.7344825125638383No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT39902.0336598742087078No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT39762.0265242255272735No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT39141.9949234956523514No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA38401.957206495479057No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC38191.946503022456906No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA34841.7757571432940193No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC32371.6498639129858612No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA23311.1880855054587711No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA7260.3700343530515092No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4870.24821863627559915No Hit
AACTTCTCCTATCCCCTCCCTACACTTCCTCCCAAATACATTCAAATTCC4830.24617987950947512No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4110.2094822577192428No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4090.2084628793361808No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3980.20285629822933976No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3460.1763524602697275No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3410.1738040143120725No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAAACAAC3180.1620811629068594No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT2970.1513776898847083No Hit
TCCCTAATCTCAAAAAACATAAACTGAGACTTGGTCTTCCCTAATCTCAA2720.13863546009643318No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2370.12079633839284803No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC2190.11162193294528995No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2120.10805410860457293No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2030.1034669058807939No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1980.10091845992313887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)