FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225767
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA115545.117665557853893No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT96834.288935052509888No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA81963.6302914066271863No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA76873.4048377309349904No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT75923.362758950599512No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC71793.179826989772642No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA67813.0035390468934784No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA67722.999552636124854No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC67612.994680356296536No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA65562.9038787776778716No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT63562.815291871708443No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA63332.8051043775219586No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT58192.5774360291805265No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA54732.4241806818534153No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA54352.4073491697192235No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG51052.2611807748696666No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT46262.049015135072885No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC44511.9715015923496348No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA43591.9307516156036975No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT41321.830205477328396No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA38411.7013115291428773No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA37861.6769501300012843No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC37261.6503740582104558No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT19210.8508772318363623No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC18100.8017114990233294No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCA17920.7937386774860808No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG17400.7707060819340293No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA12310.5452524062418334No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT12180.5394942573538205No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA11830.5239915488091705No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA10710.47438288146629043No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA10470.4637524527499591No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA10190.451350285914239No Hit
AACCCAATCTAAAACCAAAAACCTACACGTCCAATATAAAAAAAACAACT10070.4460350715560733No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA6170.2732906049156874No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT6010.26620365243813315No Hit
AACCCAATCTAAAACCAAAAACCTAAATTCTAATTTTACCTTTAAAAAAA5660.2507009438934831No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4860.21526618150571167No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT4540.20109227655060305No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4360.19311945501335448No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3420.151483609207723No Hit
CACAAAACACCTAAATTATACCCAAAAAACTAATCCCGCCGAATATCTAA3170.14041024596154442No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA3090.1368667697227673No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3060.13553796613322586No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3040.13465209707353157No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3010.13332329348399014No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2960.1311086208347544No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2820.12490753741689442No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2580.11427710870056298No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC2500.11073363246178582No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2430.10763309075285583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)