FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166226
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT63913.844765560141013No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT61293.6871488214840036No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA59803.5975118212553996No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG59293.5668307003717827No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC57343.449520532287368No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA56763.414628277164824No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA55283.32559286754178No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA55023.309951511797192No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA46742.811834490392598No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA45112.713775221686138No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC43972.6451938926521725No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA42622.5639791609014235No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA41492.4959994224730186No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT41172.4767485230950634No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC38142.294466569610049No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA36262.1813675357645614No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT34922.1007543946193734No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT31381.887791320250743No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA29791.7921384139665275No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT29161.754238205816178No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT25041.5063828763250033No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA24931.4997653796638313No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA24821.493147883002659No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC18701.1249744323992636No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA18391.1063251236268696No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA17861.074440821532131No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC15920.957732244053277No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA14560.8759159216969669No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTGCCTACCG9230.5552681289328986No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8740.5257901892604046No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT8310.49992179322127706No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT6850.41208956480935593No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA6300.37900208150349524No Hit
CCTAAAACAAACTAATACCACCCACTAAACGGAAACGAAACCCTAAAAAA4800.2887634906693297No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC4780.2875603094582075No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4740.2851539470359631No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA4460.26830941008025216No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTGCACACCCCCAAAATTA4430.26650463826356885No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC4320.25988714160239673No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA3770.22679965829653603No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3740.22499488647985275No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3370.2027360340740919No Hit
CTCCAAACTACAAAAAACTCCTTCAAGTCACCTATCCACCTCCTAAAACA2780.16724218834598678No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2700.16242946350149798No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2570.15460878562920363No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2470.14859287957359257No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2380.14317856412354263No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTCCGATT2290.1377642486734927No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT2210.13295152382900388No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2160.12994357080119837No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2000.12031812111222072No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1890.11370062445104857No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCGAATTTCTCCCTCCGT1830.11009108081768196No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA1810.10888789960655973No Hit
CACAAAACACCTAAATTATACCCAAAGAAACTAATCCCGCCGAATATCTA1810.10888789960655973No Hit
ATATAATACCCACCTTCCAAACCACAAAATAATACATCCACTAACAACTT1780.10708312778987643No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA1730.10407517476207091No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1700.10227040294538761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)