FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159247
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA72914.578422199476286No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT58663.6835858760290616No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC56843.569298008753697No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT55763.501478834766118No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA54593.428008062946241No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA52293.283578340565285No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG50223.1535915904224257No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA48683.0568864719586553No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC48433.04118758909116No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA44992.825170960834427No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA44132.7711668037702437No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC43552.734745395517655No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA43262.7165346913913604No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT39982.5105653481698242No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA36852.314015334668785No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT33602.1099298573913483No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA32352.0314354430538724No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA30441.9114959779462093No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT28131.7664383002505544No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT26261.6490106564016904No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA26251.6483827010869905No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA25181.5811914824141113No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC20531.2891922610787017No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA17451.0957820241511615No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC12200.7661054839337633No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA11960.7510345563809679No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA11130.6989142652608841No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGCCCTAACCT10820.6794476505051901No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCTAAAAAA10090.6336069125321042No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT8510.5343899728095349No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCACCTT7550.4741062625983535No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA6710.42135801616356977No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT5900.3704936356728855No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5020.3152335679793026No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA4730.29702286385300825No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA4270.26813691937681716No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4260.2675089640621174No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3070.19278228161284042No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATCCAAACCATAACGAAAAATA2870.1802231753188443No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA2680.168292024339548No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2520.1582447393043511No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2430.15259314147205286No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATTACCGTCCTAACCT2250.14128994580745632No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2180.1368942586045577No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2080.13061470545755963No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1870.11742764384886373No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1820.11428786727536468No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA1800.11303195664596508No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1770.11114809070186565No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1720.10800831412836663No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT1600.10047285035196896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)