FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238382
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC103294.332961381312347No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT99864.189074678457266No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG95954.025052227097683No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG89453.752380632765897No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT88093.695329345336477No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT86173.6147863513184717No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA85393.5820657599986574No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG85163.5724173805069177No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT84943.5631884957756874No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG75863.182287253232207No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT74803.1378208086181005No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG74653.1315283872104436No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG58882.4699851498854777No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA56322.362594491194805No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG51782.172143869923065No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG42471.7815942478878437No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA38501.6150548279651986No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG34571.4501933870845953No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT32211.3511926236041312No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT26981.1317968638571703No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATCAGTTG25861.0848134506800011No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT23830.9996560142963814No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG18550.7781627807468685No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG17600.7383107784983766No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT16120.6762255539428312No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTCC14060.5898096332776803No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTGTTTTTATTAGTTG13620.571351863815221No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG12610.5289828930036664No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGATGGTGAGG11960.5017157335704877No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG11760.4933258383602789No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTCTTACGTTTTGTTGT11360.4765460479398612No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT11220.47067312129271505No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT10160.4262066766786083No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTATGTTTTGTTGT9650.4048124438925758No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT8820.3699943787702092No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA8230.3452441879000931No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT8050.33769328221090517No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA7900.3314008608032486No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG7880.3305618712822277No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT6950.29154885855475665No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTAG6490.27225209957127633No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT6310.2647011938820884No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGCCGTTTTTTTTTA6190.25966725675596314No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT5980.25085786678524385No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTTGAGGAAATTAA4040.16947588324621826No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.14598417665763355No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG3290.13801377620793517No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTGAGGAAATTAAGG3070.12878489147670544No Hit
TTGGTGGGGAGGTTTTGATAGGTTTCGGGTTTGGGTTTAGGACGGTGAGG2950.12375095435058017No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTCAGGGGGCG2650.11116611153526691No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTAGGTAGTAGGTT2520.10571267964863118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)