FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238382
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA101044.2385750601974985No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA86133.61310837227643No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT81313.4109118977103976No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG77263.241016519703669No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA75853.1818677584716966No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT75823.1806092741901653No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA75553.1692829156563835No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC71953.0182648018726246No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA70782.969183914892903No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA67442.829072664882416No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA66772.800966515928216No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC64302.697351310082137No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC58162.4397815271287264No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT56352.363852975476336No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT52372.1968940607931806No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA51312.152427616179074No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA48252.024062219462879No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA43011.8042469649554076No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT40401.6947588324621825No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA36781.5429017291574028No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA30831.2933023466536904No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT29431.2345730801822286No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT27421.1502546333196297No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT23730.9954610666912771No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA22550.945960684951045No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA21090.8847144499165205No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC20250.8494768900336436No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA12620.5294023877641768No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAGAACGAAACCCTAAAAAA12140.5092666392596756No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA11350.4761265531793508No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC10980.4606052470404644No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTGCCG10570.4434059618595364No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAGTCCTAGGAAC9100.3817402320645015No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATCAAACAAAACGTCTAAAA8960.37586730541735536No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACGTATCCACCTCCTAAAACA8930.374608821135824No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC8830.3704138735307196No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA8010.3360153031688634No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA6860.2877734057101627No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6700.2810614895419956No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT6330.2655401834031093No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT5850.2454044348986081No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4530.19003112651122986No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4040.16947588324621826No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA3560.14934013474171706No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT3530.14808165046018576No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3210.13465781812385164No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA2970.12458994387160105No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2900.12165348054802795No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACGT2790.1170390381824131No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2550.10697116393016252No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2480.10403470060658941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)