FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313856
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC158765.058370717781402No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG138544.414126223491028No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT130414.155090232463295No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG121393.867697287928222No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT119563.8093902936378465No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA109673.49427763050571No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT105223.3524928629690045No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT104873.3413412520391517No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG101353.229187907830343No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG101303.2275948205546494No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG93192.969196064437194No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG81082.583350326264274No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG76752.4453889681892336No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT61491.9591787316476346No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA60791.9368755097879282No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG56001.784257748776509No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA53391.701098592985318No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTTATT45581.452258360522023No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT41921.3356443719412725No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTATGTTTTGTTGT39321.25280383360522No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG32601.0386929037520392No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA32141.0240365008156607No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT29790.9491613988580749No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT29520.9405587275693312No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG22040.7022328711256118No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT21020.6697338907014682No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGCATTTTTTTAAAGAG21020.6697338907014682No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGCCAGAACCTACTCGCGG20400.6499796084828712No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG19030.6063290171288744No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT18060.5754231239804242No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGCGGTGG16460.5244443311582382No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT13380.4263101549755302No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG13310.4240798327895596No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT10200.3249898042414356No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT9550.3042796696574225No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA9320.2969514681892333No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG8450.26923174959216967No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG7970.2539381117455139No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT7550.24055617862969006No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG7150.22781148042414356No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG6580.20965028548123982No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTCGGAAGTTAGAATTTATTTGTGG6190.197224204730832No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTATTT6080.1937194127243067No Hit
GGGGATTGGGATAAAGGAGTATTATCTTCGGTTTTTGTTTGAGTGATTTC5200.16568107667210438No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT5140.16376937194127242No Hit
GGTTGGAGTTTTAGGTGTATTTGGCGTAGGTTTTTTTGAGGAAATTAAGG4840.15421084828711257No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTT4810.15325499592169656No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG4780.1522991435562806No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT4690.14943158646003263No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTAAAGAGT4680.14911296900489396No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGTTGAGCGTTAGGTT4160.13254486133768353No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTAG3820.121711867862969No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGCAGTAGGAGAAGGGTT3550.11310919657422512No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.1099230220228385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)