FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235711
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT93063.948055033494406No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC83783.554352575823784No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA82453.497927546868835No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT81093.4402297728998645No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG76913.2628939676128823No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA75753.2136811604040543No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC71573.036345355117071No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA70793.0032539847525146No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA70142.975677842782051No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA67822.8772522283643953No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA67162.8492518380559244No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA61772.620581983870078No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA61142.5938543385756287No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT56402.3927606263602463No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT55432.351608537573554No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC53732.279486320112341No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT43621.8505712503871266No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT39931.6940236136624935No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA39151.660932243297937No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA36751.5591126421762243No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA32981.399171018747534No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA28711.218016978418487No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC27201.1539554793794096No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA26531.1255308407329314No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA23941.0156505211890832No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA17840.7568590350047304No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA14720.6244935535465039No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA13130.5570380678033694No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9910.42043010296507166No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA8330.35339886555994415No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA7820.3317622003215802No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT6310.26770070128250273No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6290.26685220460648845No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTC6230.2643067145784456No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA6020.2553974994802958No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA5780.24521553936812454No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5370.22782135750983196No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA5120.21721514905965353No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTCACTC5060.21466965903161075No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT5010.21254841734157504No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCTAAAAAA4990.21169992066556081No Hit
CCAAAAAAACCAACAAAAAAACTAAACAAAACCGAAAACTTCTCCATCTT4940.20957867897552512No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA4940.20957867897552512No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCCGAAAAA4890.2074574372854894No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAGCCCATTCGAA4450.18879051041317546No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT4040.1713963285548829No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT3770.15994162342869023No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG3660.15527489171061173No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3490.1480626699644904No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3340.14169894489438337No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT3250.13788070985231915No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC3140.13321397813424066No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACCC3120.13236548145822638No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTGTATTATTTGTTTCCTGA3110.13194123312021924No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT3000.12727450140214075No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2980.1264260047261265No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2980.1264260047261265No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATCAATC2850.12091077633203372No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2400.10181960112171261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)