FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226407
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT99884.411524378663204No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC87393.859862990101896No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT84593.736191902193837No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA82423.6403468090650906No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA81693.608103989717632No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA79023.490174773748161No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG75453.3324941366653857No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA73093.228257076857164No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA70623.119161510023983No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA68063.006090801079472No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT65782.9053872009257664No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA60452.669970451443639No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA58302.575008723228522No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT52622.3241330877578874No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC49432.1832363840340623No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC46792.0666322154350354No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC46002.03173930134669No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA42621.8824506309433897No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT40101.771146651826136No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT32931.454460330290141No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC29891.320188863418534No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA26051.1505828000017668No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA24451.079913606911447No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA24091.0640130384661253No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA23781.0503208823048757No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA19840.8762979943199636No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA18280.8073955310569019No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA17290.7636689678322667No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT16540.7305427835711794No Hit
CACAAAACACCTAAATTATACCCAAAAAAACCAATCCCGCCAAATATCTA11850.5233937113251799No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11530.5092598727071159No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT9560.42224842871465984No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA8320.36747980406966213No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA7570.3343536198085748No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT7310.3228698759313979No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA6230.27516817059543214No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT5880.25970928460692466No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC5850.2583842372364812No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5360.23674179685257082No Hit
ACAAACCAACCTAAATTAAAATCCCAACCCCTTAATAACCGTCCTAACCT5050.22304964069132138No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC4860.21465767401184593No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4780.21112421435732992No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAGTTTCGATT4720.20847411961644297No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4400.19434028099837902No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT4200.18550663186208907No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA4170.18418158449164557No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3840.16960606341676715No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3810.16828101604632364No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTAGCCACCTCCTAAAACA3770.16651428621906567No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3060.13515483178523632No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTGCCTACCG3000.13250473704434934No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2830.12499613527850288No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2770.12234604053761589No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT2500.11042061420362445No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2480.10953724928999545No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2420.10688715454910847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)