FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189257
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA92444.884363590250294No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT90654.789783204848433No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA75183.9723761868781606No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA71923.8001236413976764No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT69203.6564037261501556No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG67333.557596284417485No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA63543.3573394907453884No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC62993.3282784784710735No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA61813.2659293975916346No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC53362.8194465726498885No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA52952.7977829089544906No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA52082.751813671356938No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC48692.5726921593388883No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA47702.5203823372451217No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA47372.502945729880533No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT45372.397269321610297No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA42502.2456236757425088No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT36641.935991799510718No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT28141.4868670643622164No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT26001.3737933075130642No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC25671.3563567001484753No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA22791.2041826722393358No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC21221.121226691747201No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA17500.9246685723645626No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT13330.704333261121121No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT11230.5933730324373735No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT10710.5658971662871122No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA10550.5574430536254934No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA9240.48822500620848897No Hit
ACAAACCAACCTAAATTAAAATCCCAACTTCTTAATAACCGTCCTAACCT7470.3947013848893304No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA5780.3054048199009812No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCACAACGAAAAATA5360.28321277416423174No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5110.2700032231304523No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4590.24252735698019096No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAACA4310.22773265982235796No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACATCTAAAA4150.21927854716073913No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAATTATCCACGTTACTC3910.2065973781683108No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3880.20501223204425728No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA3720.19655811938263842No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAGAATA3460.18282018630750776No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3440.18176342222480543No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3350.1770079838526448No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA3130.16538357894291889No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2920.15428755607454414No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2870.15164564586778823No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2800.14794697157833No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2260.11941434134536635No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT1940.10250611602212864No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAACGAAACCCTAAAAAAA1910.1009209698980751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)