FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373312
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT185174.9601941539516545No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA156714.197829161666381No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA144433.868881793245328No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA133333.57154337390708No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT125483.361263500771473No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG125323.3569775415738046No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC124153.3256364649408536No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA119013.187950025715755No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC116123.110534887707869No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA108392.9034694839705124No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA101542.7199768558203328No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA97092.600773615635179No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT94782.538895079718841No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC92122.467641008057603No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA88522.3712069261100632No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT70901.8992156694668267No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT69391.858766929538831No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA67341.803853077318704No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA60101.6099134236242072No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA56081.5022286987827875No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT53021.4202597291273786No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC48951.311235642036688No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA46721.251500085719184No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA44871.2019436824961427No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA41291.1060453454483115No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC32650.8746035487742156No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA31970.8563882221841248No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT28040.7511143493913938No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT21550.5772651294359677No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA19950.5344055374592833No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACACTAAAAAA18300.49020658323332755No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC14770.3956476084347677No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA10960.293588205040288No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAGCCGTCCTAACCT10780.288766500942911No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTCACACACCCCCAAAATTA10590.28367692439567976No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT10590.28367692439567976No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCCATTAATC9630.2579611692096691No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA9000.24108520486884966No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCAAAAACA8550.22903094462540718No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA8350.2236734956283216No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT8250.22099477112977883No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT8180.2191196639807989No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACGG7290.1952790159437682No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA5830.1561696382650437No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA5740.15375878621635522No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA5050.13527558717641008No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4950.1325968626778673No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCCAA4800.12857877593005315No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT4420.1183996228355906No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA4390.11759600548602778No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC4210.11277430138865079No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA4020.10768472484141951No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3990.10688110749185668No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTC3860.10339876564375108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)