FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244419
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT104424.27217196699111No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA88223.609375703198196No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT82873.390489282748068No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC81843.348348532642716No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA76963.1486913865125055No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA74643.053772415401421No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA73593.0108133983037324No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA73302.9989485269148473No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA68392.7980639802961305No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA65862.6945532057655095No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG65342.673278263964749No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT63702.606180370593121No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA61702.5243536713594277No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC56672.3185595227866904No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC55752.280919241139191No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT50212.054259284261862No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC47151.9290644344343115No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT46611.9069712256412144No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA42381.7339077567619539No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA36081.476153654175821No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA32251.3194555251432991No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA28391.1615299956222715No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA28301.1578477941567553No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA27841.139027653333006No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT25311.0355168788023843No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC23600.965555050957577No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA19360.7920824485821478No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT16580.6783433366473146No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC15340.627610783122425No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA13280.5433292829117212No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA10830.4430915763504474No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA10140.4148613651148233No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG9880.4042238942144432No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT9800.40095082624509554No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA9270.37926675094816686No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA8340.34121733580449964No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCA7980.32648852994243494No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT7700.3150327920497179No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7100.29048478227961No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT6720.27493770942520834No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5880.2405704957470573No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA5460.2233868889079818No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA5280.21602248597694945No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4550.18615574075665148No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT4460.1824735392911353No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3730.15260679407083738No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3680.15056112658999504No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA3140.12846791779689795No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA3000.12274004885053943No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2780.11373911193483321No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2660.10882950998081165No Hit
TCCCTAATCTCAAAAAACATAAACTAGCTCTAAAACCAAAAATTTCGATT2620.10719297599613778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)