FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248683
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT140425.64654600435092No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC119684.8125525267107125No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA107124.307491867156179No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA105954.26044401909258No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA98333.9540298291399094No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG94713.8084629829944143No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA94073.782727407985267No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA90393.634747851682664No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA83413.354069236739142No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA78483.1558248854967967No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA74252.9857288194207086No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA71782.886405584619777No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT62682.5204778774584513No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT59702.4006466063221046No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA55382.2269314750103546No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT52932.1284124769284594No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA41721.6776377959088478No Hit
AACCCAATCTAAAACCAAAAACCTATAACCTCCTCTCATTCTCTATTAAT41541.6703996654375248No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA39721.5972141240052597No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTGGGGCCAAGAGTTTCGGTT34481.386504103617859No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA26261.0559628120941118No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT25691.0330420656015893No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAACATCCT23390.9405548429124628No Hit
CCTAAAACAAACTAATACCACCCACTAAACGGAAACGAAACCCTAAAAAA23300.9369357776768015No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA15620.628108877567023No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA7220.29032945557195305No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5370.21593755906113404No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA4980.2002549430399344No Hit
TAAAACTCACTACTATCCCCCACAACAACTACAACGAAACCCACCAAATA4780.19221257584957555No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4550.18296385358066292No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA4310.17331301295223236No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC4140.16647700084042738No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT3940.15843463365006855No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3910.15722827857151475No Hit
AACCTTTATTTAATCAAAACTACTTAATTCTAAAAACCTCCCTTCTAATA3870.15561980513344298No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA3360.13511176879802803No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2930.11782067933875658No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA2700.10857195706984395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)