FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394946
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT213745.41187909233161No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG181974.607465324373459No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA119233.0188937221797407No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT112502.8484906797384957No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA106262.6904943967023343No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT105672.6755556455819276No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA103592.622890217903207No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA88092.2304315020281256No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA86272.1843492528092447No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA84072.1286454350721367No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT77641.9658383677768607No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC73391.8582287198756284No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA72871.8450623629559484No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG61421.5551493115514525No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT60971.5437553488324987No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA56501.4305753191575556No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51491.303722534219868No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC50891.2885305839279293No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA49381.2502975090265505No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC48531.228775579446304No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC46631.180667736855165No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA45841.1606650023041125No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA45041.1404090685815276No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC44101.1166083464574903No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC42811.0839456533298222No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC34970.8854375028484907No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT34470.872777544271875No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC31980.8097309505603297No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30240.7656742947137077No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC27450.695031725856193No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT24480.6198315719110967No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA23560.5965372481301241No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC20910.5294394676740618No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC20290.5137411190390585No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16110.4079038653385526No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA16100.4076506661670203No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT15380.38942032581669395No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC14810.3749879730393522No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC14670.3714431846378999No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG13450.340552885710958No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTAACCAAAAATACTAAA13330.33751449565257025No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT12270.3106753834701453No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA12260.31042218429861296No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT12040.3048518025249021No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC11050.27978508454320333No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC10700.2709231135395725No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC10540.26687192679505556No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10010.2534523707038431No Hit
TAATCTAAACCTCCTAACCTCAAACCATAAAAAAATCATCTACCCTCATG9770.2473755905870676No Hit
CAACCATAAATACACAAAAAACAACCAAAAAAATCCTATATACCCTAATC8730.22104287674770728No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA7380.18686098859084535No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAATAATCACAC7020.17774581841568216No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAATTAACCAAAAATAAC6920.17521382670035904No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6350.16078147392301734No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT5760.14584272280261099No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT5720.14482992611648177No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5700.14432352777341714No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA5440.13774034931357704No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5010.12685278493768767No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGGCCTTAAAAAACAATACC4820.12204200067857378No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA4680.11849721227712143No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4670.11824401310558913No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCAACCTTAAAAAACAATACC4550.11520562304720139No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA4160.10533085535744126No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCTGTAGAACCATGTCGT4040.10229246529905354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)