FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302893
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA203806.728448660087887No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT172065.680553858953492No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT142124.692085984159423No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG118023.8964254703806294No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC114863.7920981996942813No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG97363.2143364158300125No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA82122.711188439481929No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT80572.6600152529110943No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA80072.643507773372115No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT76882.538190053913428No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT65482.161819520424704No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT63672.1020624444936No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG58011.9151977761123564No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT56001.8488377083656606No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT48451.599574767327076No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT48001.5847180357419948No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT47681.5741532488370482No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT39321.2981481909453174No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGTGGGGTAATAA37141.2261755801553684No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG33941.120527711105902No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA33101.0927951454804172No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT30931.0211526842812477No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT29780.9831854813415959No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG27350.9029591307821574No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT26550.8765471635197909No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTGATTAATTGAGAAGTTGTT23940.7903781203263198No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA23670.7814640813752712No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTGATTAATTGAGAAGTTGT21730.7174150607640322No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG20260.6688830709194336No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT18270.6031833023542967No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG16600.5480483206941066No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTGATTAATTGAGAAGTTGTTT15770.5206459046594012No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC15710.5186650071147236No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT13130.4334864126935915No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA12120.4001413040248537No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA10180.3360922834136147No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG10070.33246063791503927No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGTGTGAAGAAGTGG9340.3083597177881298No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG8620.28458894725199985No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT7880.2601578775343108No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTGATTAATTGAGAAGTTGTTTT7110.23473635904428294No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTGATTAATTGAGAAGTTG6620.21855902909608343No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT6280.20733394300957764No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTCTTTATTTTTTTT6020.19875005364930848No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA6010.19841990405852894No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTTATTTTAGTTATTTTGGGT5440.17960137738409274No Hit
GTTTATGAGTAGGTTTTTTTGGGGAAGGTATGTGGGGTTTAGTTTTAAGA4420.14592611912457534No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT4340.14328492239833868No Hit
GGGGATATTTAGGTTGTAAGATAGAGTGGGGTTTTAGACCAAGTCTCTGC4180.13800252894586537No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGGGTTTTTTGGGTTTTTTGAATGAGA4180.13800252894586537No Hit
GTGAAGTTTTTGGTTTTTTGTTGTAGGATAGATTTAATTAGTTTTTTATT4020.13272013549339207No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTGTA3330.10993981372960089No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT3290.10861921536648256No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT3230.10663831782180505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)