FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302893
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG192966.370566503682819No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA176345.821857883807153No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT160955.313757663597376No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA115203.8033232857807873No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA108813.592357697272634No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC97803.2288629978243137No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT85352.817826757303734No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT82772.7326481628826023No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC75172.4817344738901195No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT67132.2162942029033355No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA64222.120220671986477No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC60802.0073095119398596No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC58651.9363273499222498No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC54211.7897409316161152No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA49831.6451354108546583No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC49041.6190535931830712No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA40491.3367756930665284No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT30971.022473282644366No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30140.9950708666096607No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAGTCCCGCATCG28780.9501705222636377No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA27780.9171555631856794No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA25480.8412211573063756No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCACAAAAC24440.806885599865299No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA24350.8039142535482827No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC22700.7494395710696516No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG21630.7141135648562363No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA21230.700907581225053No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA18700.6173797347578187No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG18220.6015325544003988No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT14940.4932434886246959No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14520.4793772058119534No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA14370.4744249619502597No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC13660.4509843410049093No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT13640.4503240418233502No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC12210.40311265034186994No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA11990.3958493593447191No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10470.3456666215462226No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC9860.3255274965086681No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG8300.2740241603470533No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7560.24959309062936416No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6230.20568319505567972No Hit
AATACCTAAAAACCCAAAACCCTTCTCCGTTTTAAAAAAAAAACAACATT6070.2004008016032064No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAAATAACTAAATTAACC5910.19511840815073309No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA5670.18719481797202314No Hit
AAACTACAAAATCAAAACCAACCCAACTAAATAAAAACTTACCCAACCCC4980.16441449620823195No Hit
TAAAACTAAACCCCACATACCTTCCCCAAAAAAACCTACTCATAAACTGT4820.15913210275575865No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4680.15451000848484447No Hit
TACTATCCACACTCTCTTACCTCCCCTAACAACCCTATCCTCTACAAATT4140.13668193058274702No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4050.1337105842657308No Hit
CTCATTCAAAAAACCCAAAAAACCCAACTTTACTACTCCCTTTTCCCTTT3810.12578699408702085No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA3220.10630816823102547No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA3150.10399712109556841No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC3060.10102577477855215No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3030.10003532600621343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)