FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347629
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG172594.964775666011754No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT138023.970324685224765No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT118093.3970123321126833No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA81642.348480707881103No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT80792.3240293531322185No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA72352.081241783625647No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA69972.01277799032877No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC69622.0027097854321703No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC62271.7912774826035802No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC59751.7187864073480634No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA55141.5861737657099955No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC53641.5430243161531403No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC49431.4219181943969001No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA48771.4029324365918838No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA46431.3356192952831898No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA46181.3284277203570474No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC41261.1868975258105623No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG39641.1402961202891588No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA38641.1115298205845887No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT37011.0646407520661394No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT33990.9777665269583378No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA33380.96021908413855No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA33140.9533151722094532No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA32460.9337540884103456No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA32100.9233982205167003No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA31920.9182202865698776No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC28970.8333597024413959No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA26400.7594303122006507No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA26370.7585673232095136No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC26350.7579919972154222No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA24900.7162808626437955No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC23390.6728437500898947No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT22560.6489677213351015No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG21100.6069689237664292No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA19690.5664084411829853No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC17870.5140537757206677No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC17240.4959310069067886No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA15950.4588224802878931No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA14980.43091916957446014No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT14310.4116457487723981No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT14170.40761846681375835No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13570.3903586869910163No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA12970.3730989071682742No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12730.36619499523917737No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC12300.35382548636621225No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA11560.33253842458483035No Hit
CCCAAAAATTAATAAATAAACCCCATGTCGTCAGTGAACCACTCACCTAC11340.32620983864982495No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA10780.31010071081526575No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCGCCAAACCCCCGCAAAAC8660.24911615544157706No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8570.24652718846816576No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA7830.2252401266867839No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT7720.22207583371928122No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA7520.21632257377836717No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7380.21229529181972734No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC7190.20682969487585903No Hit
CAACTACCAAACCCTAAAAAACCACATAAACAAAACTATCTCCAAACCTA7140.20539137989063053No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA7040.20251474992017351No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC7040.20251474992017351No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC7030.20222708692312782No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA6870.19762447897039662No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6430.18496730710038575No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCACAAAAC5940.1708718202451464No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5520.15878997436922698No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACAACCTTAAAAAACAATACC5130.14757111748444462No Hit
ATAATACTCCTTTATCCCAATCCCCTACCCCATATTTTATAAATCCCTAT4890.14066720555534779No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC4770.1372152495907994No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4730.13606459760261658No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4660.13405095662329666No Hit
TAAAACTAAACCCCAAAAAAACCTACTCATAAACTGTAGAACCATGTCGT4550.13088666365579396No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCGCCCCCTAA4490.12916068567351977No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4440.12772237068829126No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4410.12685938169715416No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT3920.11276389484191479No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC3910.11247623184486909No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA3890.1119009058507777No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT3810.10959960187441209No Hit
ATAATACTCCTTTATCCCAATCCCCATCCTCAACAATAAACCCTCAATAC3730.10729829789804647No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA3680.10585998291281797No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3570.10269568994531526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)