FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306799
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG141254.6039915384339585No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT121563.9622032666338547No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT104083.3924491279306648No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT83552.723281366627662No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA78972.5739979595761393No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA70572.3002030645471465No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC62062.022822760178488No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA60591.9749086535484146No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC52591.7141516106636594No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC52561.7131737717528412No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA51871.6906834768040315No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51621.6825348192138827No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC50141.6342947662802028No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT46821.5260805934830295No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC45571.4853373055322865No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG39381.2835765436002073No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA38731.262390033865821No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG31901.0397687085029612No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC30771.0029367761954895No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT29910.9749053940853785No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC28780.9380734617779067No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA28500.9289469652769402No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT28490.9286210189733344No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA27960.9113458648822194No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC27280.8891815162370151No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA26970.8790771808252308No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA25890.8438749800357889No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA24800.808346832942741No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA24740.8063911551211054No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA24270.7910716788516259No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA21800.7105629418609578No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC18490.6026747153673904No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA18070.5889849706159407No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA17400.5671465682743425No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA15920.5189065153406628No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15110.4925048647485813No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT15090.4918529721413694No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC14780.4817486367295852No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTGGCCCCGTTAACTCCC14730.4801189052115555No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA14070.45860644917356314No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA13500.4400275098680243No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC13390.43644210052835897No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA12330.4018917923461289No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT12230.3986323293100695No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA11640.37940149739731877No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT11480.3741863565396237No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC11380.3709268935035642No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11130.3627782359134156No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC10590.34517713551869467No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA10550.3438733503042709No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAAGGAAAAAAATAATCACA9720.31681980710497754No Hit
ATAATACTCCTTTATCCCAATCCCCTAAATCAATTACCTCCATTAAAACA9660.3148641292833419No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA9560.31160466624728245No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC9340.3044338475679516No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8360.27249110981456914No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA7840.25554190202706006No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA7560.24641540552609362No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAACCCTATAACTCTAAA7410.24152621097200447No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA6800.2216434864520419No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC6790.22131754014843597No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC6730.21936186232680027No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATGCTAAA5950.19393805064553665No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5520.17992235959048108No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT5220.17014397048230276No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAACCCTATAACTCTAAAA4770.15547638682003528No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA4470.14569799771185693No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT4400.14341637358661535No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAACCCAATTCACCCAT4150.13526771599646675No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT4060.13233419926401324No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4020.13103041404958946No Hit
TCCAACCAATAACACTATCTCTAACCATGTCGTCAGTGTCCTAAACCTAT3920.12777095101353003No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3670.11962229342338143No Hit
TAAAACTAAACCCCAAAAAAACCTACTCATAAACTGTAGAACCATGTCGT3540.11538499147650416No Hit
TACTATCCACACTCTCTTACCTCCCCCACTTTCACTTCCCGTAACCTCCA3170.10332497824308423No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC3140.10234713933226641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)