FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350400
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA243706.9549086757990874No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGATTTTTTGT149324.261415525114155No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTGTC143104.083904109589041No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT139133.9706050228310503No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGATTTTTTGT127573.6406963470319638No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA121583.4697488584474883No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTCGGGGCGCAGGCGGCGTTGAT98892.822203196347032No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG88692.5311073059360734No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA88262.518835616438356No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT86962.4817351598173514No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT84772.4192351598173514No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT80512.297659817351598No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGCGGGGGGTAT71822.0496575342465753No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG71792.0488013698630136No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT71082.028538812785388No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGCGGCGTTGAT69381.9800228310502284No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA57721.6472602739726026No Hit
GGGGATATTTAGGTTTGTTTTTTTTCACTGACGACATGGTTCTACAGGGG47551.3570205479452055No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT44541.2711187214611872No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT43421.2391552511415524No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT31000.8847031963470319No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG24010.685216894977169No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT20350.5807648401826484No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT18780.535958904109589No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT17790.5077054794520548No Hit
GGGGATATTTAGGTTGTAAGATAGAGTGGGGTTTTAGACCAAGTCTCTGC17630.5031392694063928No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA15790.4506278538812785No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA15580.44463470319634707No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC13150.3752853881278539No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT11690.3336187214611872No Hit
GTTTATGAGTAGGTTTTTTTGGGGTCGTGTAGAGGGTTCGTTGGGGTTTT10240.2922374429223744No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTGTCG9870.2816780821917808No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT9600.273972602739726No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA8710.2485730593607306No Hit
GTTTATGAGTAGGTTTTTTTGGGGAAGGTATGTGGGGTTTAGTTTTAAGA8590.245148401826484No Hit
GGGGATATTTAGGTTTGTTTTTTTTCACTGACGATATGGTTCTACAGGGG7490.2137557077625571No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT7330.209189497716895No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT7030.20062785388127855No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG6640.18949771689497716No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT6490.18521689497716895No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTATGGGATAGGGAGTATTT6140.1752283105022831No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA5900.16837899543378995No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT5890.16809360730593606No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTATATTTTAGAGGTAGTATTATT5680.16210045662100458No Hit
TTAGGGTGGAAGATAGAGATTTGTGTCTATAGGGTTTAGGAAGGCGGGTA5680.16210045662100458No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA5080.1449771689497717No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT5040.14383561643835616No Hit
TGAGGGTAGATGATTTTTTTATGGTTTGAGGTTAGGAGGTTTAGATTAAG4540.1295662100456621No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT4150.11843607305936073No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGATTTTTGTA4150.11843607305936073No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGATTTTTGTA3860.11015981735159819No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT3820.10901826484018265No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC3640.1038812785388128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)